Bài báo khoa học

The objective of this study is to assess the differentiation of blood cell lines following the transplantation of CD34+ hematopoietic stem cells. These stem cells, found in the bone marrow, have the ability to develop into various types of blood cells. Normally, they are primarily located in the bone marrow, with only a small number present in the peripheral blood. However, in response to injury, stress, or chemical infection, these cells migrate to the peripheral blood. When high doses of chemotherapy are administered, rapidly dividing cells, including hematopoietic cells, are affected, resulting in a reduction in their quantity. To expedite the restoration of blood cell lines, CD34+ autologous hematopoietic stem cell transplantation is performed prior to high-dose chemotherapy in cancer treatment. Methods: CD34+ cells were extracted from the bone marrow of cancer patients using G-CSF and filtered using a COBE spectra cell counter. The stem cells were preserved in liquid nitrogen, after which their viability was assessed using a Faccalibur cytometer. Results: G-CSF successfully mobilized CD34+ cells in cancer patients, with an average age of 40.31 ± 8.45 years and a mean mobilization time of 6.91 ± 1.43 days. The number of CD34+ cells in the peripheral blood increased by 16.78 times following mobilization. The survival rate of CD34+ cells after chemical treatment was 89.72%, after awakening from liquid nitrogen was 79.82%, and after 30 minutes of awakening, it was 54.50%. Following transplantation of an average of 107.71 ± 11.5 CD34+ cells per patient, the earliest differentiation was observed in neutrophils on day 9 and platelets on day 10. The hematopoietic cells achieved stability in terms of differentiated cell lines after 3 months.