Pigments, Phenolics, Antioxidant Activity, Anti--Amylase, and In Vitro AntiInflammatory Properties of Crude Acetonic Extract from Viola dalatensis
Authors: Pham M. Tuan, Nguyen T. Ngan, Nguyen X. Ha, Hoang V. Trung
Tropical Journal of Natural Product Research
: 7(12):5606-5610 : 5606-5610
Publishing year: 1/2024
Viola dalatensis Gagnep. is a member of the Violaceae family. Viola plants are commonly used in traditional medicine. Limited data on the chemical and biological activities of this species are found in the literature. This study aimed to identify the pigments and phenolic compounds in the aerial parts of the plant. In addition, the in vitro antioxidant, �-amylase inhibitory and anti-inflammatory properties of
the acetonic extract of the plant were evaluated. The level of chlorophyll a in the dry aerial parts of V. dalatensis was twice as high as that of chlorophyll b. Among the phenolic compounds examined, kaempferol was the most abundant compound, with an average concentration of 16.21 mg/100 g dry weight. The antioxidant activity of the extract determined by ABTS and DPPH assays was weaker than that of ascorbic acid. The in vitro anti-inflammatory efficacy of the extract was assessed by its ability to inhibit albumin denaturation (IC50 = 0.17 ± 0.01 mg/mL) which was slightly lower than that of diclofenac (IC50 = 0.09 ± 0.00 mg/mL). The anti-�-amylase activity of the extract (IC50 = 0.80 ± 0.01 mg/mL) was slightly stronger than that of acarbose (IC50 = 0.92 ± 0.01 mg/mL). These results provide a better understanding of the phytochemical constituents of V. dalatensis and its potential health-promoting activities.
Viola dalatensis, kaempferol, antioxidant, amylase, albumin denaturation