SPECIES IDENTIFICATION OF LEPTOBOTIA PELLEGRINI FANG,
1936 IN NGHE AN USING DNA BARCODE
Authors: Le Dinh Chac, Hoang Ngoc Thao, Nguyen Thi Yen, Ong Vinh An, Le Anh Son
TNU Journal of Science and Technology
: 229(01): 486 - 493 : 486 - 493
Publishing year: 12/2023
Leptobotia pellegrini Fang, 1936, is an ornamental and high
commercial value species. In Vietnam, this species was recorded in
2005 by Nguyen Van Hao in Hong River with the name Leptobotia
elongata, and was re-identified as Leptobotia pellegrini (Kottelat:
2012, 2013). In 2022, Hoang et al. Discovered this species in Tuong
Duong, Nghe An, described ist morphological characteristics, and
identified this as a new distribution area of L. pellegrini in Vietnam.
In this study, we used molecular taxonomy research methods to obtain
ba DNA barcode loci: Cybt, ND2 and 16S to identify L. pellegrini in
Vietnam. The sizes of sequences of the Cybt, ND2, and 16S we
obtained respectively are Cytb 818 bp, ND2 512 bp and 16S 750 bp.
Samples of the same species, L. pellegrini, are placed in the same
group on the phylogenetic tree with high bootstrap coefficients, from
99%, 100%, and 89% based on Cytb, ND2 and 16S gene sequences.
This has contributed to the analysis, description of morphological
characteristics and new records of L. pellegrini species in Vietnam.
Cybt ND2 16S Leptobotia pellegrini Loach