OPTIMIZATION OF EXTRACTION OF TOTAL PHENOLIC CONTENT AND
ANTIOXIDANT ACTIVITIES FROM Ganoderma lucidum (Leyss ex. Fr.) Karst.
MUSHROOM AT NGHE AN, VIETNAM
Authors: Nguyen Tan Thanh, Le Thi My Chau, Dao Thi Thanh Xuan, Tran Phuong Chi, Nguyen Thi Huyen, Dinh Thi Kim Hao, Le Thi Mai Trang, Dinh Thi Phuong An, Nguyen Duc Dien
: : 481-486
Publishing year: 10/2022
Ganoderma lucidum (Leyss ex. Fr.) P. Karst, a rich source of several bioactive compounds, has been utilized for its medicinal and nutraceutical purposes since earliest history. Among its bioactive constituents, the total phenolic content and antioxidant activities have been widely reported to be responsible for some its bioactive properties.
Recent trends in bioactive compounds’ recovery have been focused on finding the best techniques that maximally extract valuable compounds from natural matrices. This study is aimed at optimizing the extraction of total phenolic content (TPC) and antioxidant activities (DPPH) from G. lucidum using microwave assisted extraction (MAE) techniques. Response surface methodology (RSM) was applied using a circumscribed central composite design with three independent variables (microwave power, time extraction and ethanol concentration). The yield
of TPC and DPPH were maximized and the optimal conditions were determined. The conditions that maximize the responses (total phenolic content and antioxidant activities) were: microwave power 200 W, extraction time 22 min and ethanol concentration 71% ethanol. The experimental values of TPC and DPPH radical scavenging activities were 30.05±0.5 mg GAE/g and 94.82±0.95%, respectively
Antioxidant activities, Ganoderma lucidum, microwave-assisted, phenolic, response surface methodology.
