Research on creating emulsion from antibacterial compound of eucalyptus camaldulensis for prevention treatment of hepatopancreatic necrosis in white shrimp
Authors: Le Minh Hai, Mai Thi Minh Ngoc, Đoan Quoc Hung
Journal for Rural Development & Rural Developmen
: 20 : 85-93
Publishing year: 8/2020
Acute hepatopancreatic necrosis disease (AHPNS) caused by V. parahaemolyticus is the main cause affecting the sustainable development of the shrimp farming industry. The study was conducted in order to find out the optimal specifications Eucalyptus essential oil extraction, assessing the antibacterial ability of essential oil and Eucalyptus essential oil to two strains of bacteria V. parahaemolyticus DHM-03.18, V. parahaemolyticus DHM-04.19 causing acute hepatopancreatic necrosis in shrimp cultured in vitro laboratory. The results show that the extraction factors, affecting the efficiency of extracting eucalyptus essential oil and the suitable conditions for distillating eucalyptus oil by steam attraction method are: materials/water ratio of 1/7, 140°C, 4.5 hours. The use of eucalyptus oil to inhibit AHPNS causing vibrio in the testing shows a positive result in which the resistance is positively correlated with the antimicrobial activity of esensial oil. The lowest obtained sterile ring diameter was 9.35 mm, corresponding to the amount of 25 ul of essential oil and the highest diameter of sterile ring was 23.55 mm with an herbal concentration of 100 ul. Using white eucalyptus oil emulsion to treat AHPNS on white shrimp showed that the mortality rate decreased and still developed well.