Bài báo khoa học

Nervous necrosis is an acute disease that causes mass mortality of grouper in the juvenile, fingerlings adult stages. This paper showed the results of a research to develop an inactivated vaccine to prevent nervous necrosis disease for farmed grouper in a laboratory scale. TB05, a virus strain causing nervous necrosis disease was isolated from Thai Binh province, was selected as the strain for development of an inactivated vaccine; the virulence of strain TB05 on cells and fingerlings is TCID50 = 106,8 and LD50 = 107,5, respectively. The vaccine was produced by growing strain TB05 in GS01 cells and then inactivated with beta - propiolactone 0.1% and adsorbed with aluminum hydroxide. By the immersion method, vaccine was safe for grouper from larvae stage to fingerlings 1.5 cm in length. The relative protection rate of vaccine reached 75% with the challenge dose 0.2 x TCID50,0.5 x TCID50 and 1 x TCID50. This results provide a scientific basis for the production of vaccine to prevent grouper from nervous necrosis disease, helping to sustainably develop grouper farming in Vietnam.