Bài báo khoa học

The fungus entomopathogenic Isaria tenuipes were collected in Pu Mat National Park, Nghe An province. The synnemata of I. tenuipes were successfully cultured on silkworm pupae when inoculated with at a concentration of 106 cfu/ml and light intensity of about 300 lux, giving the infection rate of silkworm pupae to 70.00 ± 3.55% and 68.80±4.12% formed synnemata; average synnemata weight 1.68 g/pupae. I. tenuipes develops on silkworm pupae in 2 cycles. Cycle 1 formed synnemata and conidia, the life cycle 40.08 ± 2.50 days. Cycle 2 only formed conidia, the life cycle 19.52 ± 1.53 days. Research on infection mechanism and development of I. tenuipes on silkworm pupae is of great significance in biological control of plant insect pests and medicinal applications.